Tools

The CTSF has available an High Content Screening (HCS) system for a variety of screenings and other large scale experiments. The HCS system includes incubation, robotics and liquid handling for screenings in living cell systems. In addition, the CTSF has two small molecule compound libraries that can be used in the HCS screening facility.

Temporal data storage is in collaboration with the CCBC.

Equipment

Opera PhenixTM HCS system (Perkin Elmer)
  • Confocal SynchronyTM Optics
  • Objectives
    • 5x Air, NA 0.16, long WD
    • 10x Air, NA 0.3, long WD
    • 20x Air, NA 0.4, WD 8.28 mm
    • 40x water immersion obj. NA 1.1
  • Cameras
    • 16 bit sCMOS 4.4 megapixel (6.5 μm pixel size) (2x)
  • Imaging modes
    • Fluorescence, brightfield and digital phase contrast imaging
  • Laser lines
    • 405, 488, 561, 640 nm solid state lasers
  • Filters
    • 435-480, 435-515, 435-550, 500-550, 570-630 and 650-760 nm
  • Sample holders
    • Multiwells (96/384) plates, slides (4x)
    • (Other sample types can be discussed)
Automation and liquid handling
  • Robotics
    • Plate::handler workstation SX 2
  • Incubator
    • 40 plate incubator (temp: 25-50C, RH up to 95%, CO2 0-5%)
  • Dispensor
    • Janus 4-tip
  • Washer
    • ELx405 (50 - 3000 μL/well)
  • Barcode reader

Libraries

  • LOPAC1280(library of 1280 pharmacologically active compounds) (LO1280 Sigma).

Data analysis/management

Available software within the CTSF:

Harmony® High Content Imaging and Analysis Software provides a complete solution to set up assays and automate your high content imaging experiments, acquire images and analyze data, and then store, retrieve and present the results.

The PhenoLOGIC™ machine-learning option enables our High Content Analysis software to recognize different cell populations and regions using a simple learn-by-example approach. PhenoLOGIC sets parameters for optimal image segmentation and cell classification. The software combines the most meaningful parameters, whether it’s two, three, four or more, to achieve accurate classification of cells. The result is highly robust and statistically relevant results.

Columbus™ Scope system is a universal high-volume image data storage and analysis system that rings access to images from a wide range of sources including all major high content screening instruments via the Internet.

ImageJ/Fiji is a public domain Java image processing and analysis package. Customized image analysis can be performed in collaboration with Erasmus Optical Imaging Centre (OIC) and Biomedical Imaging Group Rotterdam (BIGR).

Download image analysis software: